This table presents a detailed characterization of the IS elements and
associated ORFs in the Shigella flexneri 5a invasion-associated
plasmid pWR501,
and constitutes a supplement to the analysis of the plasmid sequence as
described by Venkatesan,
et al. The table lists (a) known IS elements,
(b) new IS elements, and (c) unknown IS-related
ORFs. For further information on known IS elements see the
IS Database.
Each ORF in pWR501 is referred to by its unique identifier (ID; S number),
as assigned in the sequence annotations. In the table, ORFs constituting
complete IS elements are indicated in bold. Abbreviations used:
aa = amino acids, hp = hypothetical protein, id = identical, GB = GenBank
accession no., IR = inverted repeat, DR = direct repeat, tnp = transposase,
URF = unidentified open reading frame.
Name and description of IS element |
pWR501 ORF ID |
size (aa) |
homology to protein in database |
additional comments regarding similarity |
IS1
S. dysenteriae iso-IS1 (NuXi), 803 bp [GB:
J01737]
InsA, orfA: 90 aa
InsB, orfB: 131 aa
S. boydii IS1SB, 768 bp [GB: U96388]
InsA, orfA: 91 aa
InsB, orfB: 167 aa
(InsAB' translational fusion protein via frameshift at A6C
motif) |
S0037 |
122 |
86% id to NuXi InsB over 122 aa |
homology to NuXi bp 431-778 |
S0053 |
75 |
96% id to IS1SB InsA |
S0053-S0054 = complete IS1 with 9 bp target
site duplication (CCTCGATAC) |
S0054 |
167 |
98% id to IS1SB InsB |
|
S0067 |
75 |
96% id to IS1SB InsA over 75 aa + LIR |
|
S0068 |
152 |
86% id to IS1SB InsB over 145 aa |
missing the terminal 87 bp of 768 bp IS1 seq |
S0082 |
94 |
71% id to NuXi InsA over 81 aa |
|
S0083 |
61 |
84% id to NuXi InsB over 56 aa |
|
S0226 |
62 |
80% id to NuXi InsB |
S0226-S0227 homology to NuXi bp 183-522 |
S0227 |
55 |
96% id to 33 aa of IS1-cat |
IS1-cat protein fragment, 33 aa [GB:
M24180] |
S0228 |
167 |
98% id to IS1SB InsB |
S0228-S0229 = complete IS1 with no target site
duplication |
S0229 |
92 |
IS1SB InsA |
|
S0239 |
167 |
IS1SB InsB |
S0239-S0241 = complete IS1 with 8 bp target
site duplication (CACTATCG) |
S0240 |
92 |
IS1SB InsA |
|
S0241 |
70 |
77% id to IS1 hp |
IS1 hp, 70 aa [GB: BAA01301], overlaps InsA (S0240) |
S0284 |
91 |
79% id to NuXi InsB over 48 aa |
homology to NuXi InsB aa 76-131 |
Total IS1 ORFs: 15, complete
elements: 3 |
|
IS2
E. coli IS2, 1330 bp
orf1, TnpG: 136 aa [GB: BAA15011]
orf2, TnpF: 301 aa [GB: BAA15010] |
S0001 |
101 |
88% id to orf2 over 85 aa |
homology to IS2 bp 1-36, 52-393 |
S0017 |
39 |
56% id to orf2 over 41 aa |
homology to IS2 bp 4-227 |
S0052 |
95 |
71% id to orf2 over 91 aa |
homology to IS2 bp 53-235 |
S0106 |
301 |
99.7% id to orf2 |
S0106-S0107 = complete IS2 with 5 bp target
site duplication (GGACA) |
S0107 |
136 |
100% id to orf1 |
|
S0220 |
283 |
99% id to orf2 over 240 aa |
S0220-S0221 = incomplete IS2, bp 197-1284 |
S0221 |
151 |
98% id to orf1 over 136 aa |
|
S0222 |
301 |
99% id to orf2 |
S0222-S0223 = complete IS2 with 5 bp target
site duplication (TGAGG) |
S0223 |
136 |
94% id to orf1 |
|
S0224 |
72 |
63% id to orf1 over 48 aa |
|
Total IS2 ORFs: 10, complete
elements: 2 |
|
IS3
E. coli, 1258 bp [GB: X02311]
orfA/hp, TnpA: 102 aa [GB: BAA97886]
transposase: 288 aa [GB: BAA97887] |
S0026 |
71 |
90% id to transposase over 50 aa |
homology to IS3 bp 1062-1258 |
S0033 |
195 |
96% id to transposase over 184 aa |
homology to IS3 bp 672-1258 |
S0174 |
121 |
86% id to transposase over 116 aa |
S0174-S0175 shows homology to IS3 bp 1-668 |
S0175 |
102 |
100% id to orfA/hp |
|
S0188 |
80 |
90% id to orfA/hp over 80 aa |
homology to IS3 bp 403-776 |
S0248 |
73 |
97% id to transposase over 51 aa |
homology to IS3 bp 452-517, 530-791 |
S0291 |
73 |
88% id to transposase over 50 aa |
homology to IS3 bp 1074-1258, 1215-1258 |
Total IS3 ORFs: 7 |
|
IS4
E. coli, 1426 bp [GB: J01733]
orf: 442 aa [GB: AAA61828] |
S0022 |
442 |
99% id |
10 bp target site duplication (CCGGCGGCCT) |
S0243 |
422 |
97% id |
homology to IS4 bp 1-1337 |
Total IS4 ORFs: 2, complete elements:
1 |
|
IS21
P. aeruginosa, 2131 bp [GB: X14793]
IstA: 391 aa
IstB: 266-272 aa |
S0018 |
75 |
88% id to IstB over 43 aa |
homology to IS21 bp 1824-1941, 1951-2098 |
Total IS21 ORFs: 1 |
|
IS91
E. coli, 1829 bp [GB: X17114]
transposase, orfb: 426 aa [GB: CAA34970]
orfa, orf121: 133 aa [GB: CAA34969] |
S0019 |
133 |
88% id to orfb over 60 aa |
S0019-S0020 shows homology to IS91 bp 191-522,
followed by CCGG, then to bp 1224-1829 |
S0020 |
62 |
90% id to orfa over 60 aa |
|
S0184 |
133 |
88% id to orfb over 60 aa |
S0184-S0185 shows homology to IS91 bp 1-522
followed by CCGG, then to bp 1224-1829 |
S0185 |
140 |
94% id to orfa over 120 aa |
|
Total IS91 ORFs: 4 |
|
IS100
Y. pestis, 1954 bp [GB: Z32853] IS21 family
orfB, orf11: 259 aa [GB: CAA21334]
orfA, orf12: 340 aa [GB: CAA21335] |
S0035 |
144 |
93% id to orfA over 118 aa |
S0035-S0036 shows homology to IS100 bp 756-1954 |
S0036 |
221 |
99% id to orfB over 220 aa |
|
S0050 |
107 |
98% id to orfA |
S0050 region shows homology to IS100 bp 1172-1486 |
S0089 |
77 |
83% id to orfB over 77 aa |
S0089 region shows homology to IS100 bp 1241-1445 |
S0094 |
221 |
100% id to orfB over 220 aa |
S0094 region shows homology to IS100 bp 1-61,
1173-1954 |
S0129 |
340 |
100% id to orfA |
S0129 region shows homology to IS100 bp 1-1051 |
Total IS100 ORFs: 6 |
|
IS150
E. coli, 1443 bp [GB: X07037]
orfA: 174 aa [GB: CAA30085]
orfB: 284 aa [GB: CAA30086] |
S0046 |
135 |
97% id to orfB over 100 aa |
S0046 region shows homology to IS150 bp 800-1099 |
S0048 |
81 |
96% id to orfB over 79 aa |
S0048-S0049 shows homology to IS150 bp 1-804 |
S0049 |
174 |
100% id to orfA |
|
Total IS150 ORFs: 3 |
|
IS600
S. sonnei, 1264 bp [GB: X05952]
orfA, TnpJ: 100 aa [GB: CAA29384]
orfB, TnpI: 272 aa [GB: CAA29385] |
S0009 |
140 |
91% id to orfB over 116 aa |
S0009-S0010 region shows homology to IS600 bp
1-743 |
S0010 |
101 |
92% id to orfA |
|
S0060 |
63 |
90% id to orfB over 63 aa |
S0060-S0061 region shows homology to IS600 bp
44-569 |
S0061 |
101 |
orfA |
|
S0108 |
273 |
orfB |
S0108-S0109 region shows homology to IS600 bp
1-1244, missing inverted repeat |
S0109 |
101 |
orfA |
|
S0123 |
101 |
94% id to orfA |
S0123-S0124 = complete IS600 |
S0124 |
236 |
92% id to orfB over 235 aa |
|
S0168 |
250 |
91% id to orfB over 229 aa |
S0168 region shows homology to IS600 bp 408-1098 |
S0169 |
273 |
orfB |
S0169-S0170 = complete IS600 |
S0170 |
101 |
orfA |
|
S0171 |
101 |
orfA |
S0171 region shows homology to IS600 bp 1-408 |
S0172 |
63 |
95% id to orfB over 62 aa |
S0172 region shows homology to IS600 bp 1012-1264 |
S0201 |
67 |
97% id to orfB over 61 aa |
|
S0255 |
273 |
orfB |
S0255-S0256 = complete IS600 |
S0256 |
101 |
orfA |
|
S0266 |
273 |
orfB |
S0266-S0267 region shows homology to IS600 bp
183-1219; Tn501 insertion site begins immediately upstream
of IS600 bp 183 |
S0267 |
42 |
98% id to orfA over 42 aa |
|
S0275 |
60 |
92% id to orfA over 42 aa |
S0275 region shows homology to IS600 bp 1-189,
bp 183-189; (CCTAAAG) appears to be the target site for insertion
of Tn501 |
S0285 |
100 |
94% id to orfA |
S0285-S0286 region shows homology to IS600 bp
1-588; immediately upstream is an IS600 sequence from bp 1157-1264 |
S0286 |
62 |
90% id to orfB over 62 aa |
|
Total IS600 ORFs: 21, complete
elements: 3 (an additional complete element is disrupted by Tn501) |
|
IS629
S. sonnei, 1310 bp [GB: X51586]
TnpC: 296 aa [GB: CAA35936]
TnpE: 108 aa [GB: CAA35935]
S. flexneri, 1306 bp [from GB: AF141323]
(Note: four bases from the S. sonnei IS629 sequence
(bp 579-582) are deleted in the S. flexneri IS629)
TnpC: 206 aa [GB: AAD44736]; corresponds to aa 91-296 of S. sonnei TnpC
TnpD: 118 aa [GB: AAD44737]
TnpE: 108 aa [GB: AAD44738]
|
S0043 |
223 |
98% id to S. sonnei TnpC over 222 aa |
S0043-S0045 = complete IS629, no target site
duplication |
S0044 |
119 |
TnpD |
|
S0045 |
109 |
TnpE |
|
S0057 |
108 |
TnpE |
S0057-S0059 = partial IS629, homology to IS629
bp 1-410, bp 507-1310 |
S0058 |
83 |
100% id to TnpD over 74 aa |
|
S0059 |
223 |
98% id to TnpC over 223 aa |
|
S0069 |
108 |
TnpE |
S0069-S0071 region = partial IS629, similar
to that seen at S0057-S0059 |
S0070 |
84 |
TnpD |
|
S0071 |
215 |
98% id to TnpC over 215 aa |
|
S0086 |
108 |
TnpE |
|
S0091 |
263 |
98% id to S. flexneri TnpC over 193 aa |
S0091-S0093 region has a complete IS629 sequence,
but TnpC stop codon is beyond the right inverted repeat of IS629
due to a -1 frameshift; no target site duplication |
S0092 |
119 |
TnpD |
|
S0093 |
109 |
TnpE |
|
S0096 |
85 |
87% id to TnpE |
|
S0180 |
109 |
TnpE |
S0180-S0182 = complete IS629 with 3 bp target
site duplication (TTC) |
S0181 |
118 |
TnpD |
|
S0182 |
206 |
TnpC in S. flexneri |
|
S0189 |
187 |
100% id to TnpC over 187 aa |
|
S0190 |
72 |
90% id to TnpE over 64 aa |
|
S0195 |
108 |
TnpE |
S0195-S0197 = complete IS629, no target site
duplication |
S0196 |
106 |
TnpD |
|
S0197 |
215 |
TnpC in S. flexneri |
|
S0244 |
109 |
TnpE |
S0244-S0246 = complete IS629 with 3 bp target
site duplication (AAG) |
S0245 |
118 |
TnpD |
|
S0246 |
223 |
TnpC |
|
S0287 |
122 |
99% id to TnpC over 115 aa |
|
S0293 |
55 |
94% id to TnpE over 35 aa |
|
Total IS629 ORFs: 27, complete
elements: 5 |
|
IS630
S. sonnei, 1153 bp [GB: X05955, bp 4-1156]
orf: 343 aa [GB: CAA29389] |
S0029 |
47 |
78% id to IS630 orf over 45 aa |
homology to IS630 bp 269-323 and bp 999-1153,
with 15 bp in between lacking homology |
S0080 |
344 |
99.4% id to IS630 orf |
S0080 = complete IS630 with 2 bp target site
duplication (TA) |
S0254 |
318 |
97% id to IS630 orf over 313 aa |
homology to IS630 bp 209-1153 |
Total IS630 ORFs: 3, complete
elements: 1 |
|
IS911
S. dysenteriae, 1250 bp [GB: X17613] IS3 family
orfA: 120 aa
orfB: 273 aa
|
S0288 |
113 |
96% id to orfA over 112 aa |
S0288-S0289 = complete IS911 with one base missing
at IS911 bp 1209; no target site duplication |
S0289 |
280 |
99% id to orfB over 273 aa |
|
Total IS911 ORFs: 2, complete
elements: 1 |
|
IS1294
E. coli plasmid pUB2380, 1688 bp
tnp1294: 351 aa [GB: CAA07835]
translational fusion protein: 389 aa
(Note: sequence of IS1294 is from plasmid pUB2380 [GB:
AJ008006, bp 5401-7088]; in the GenBank entry for the E. coli
IS1294 sequence itself [GB: X82430] the IS1294 element is from bp 2100-3787, not from bp
363-2011 as annotated)
|
S0047 |
399 |
98% id to 345 aa |
complete IS element; target site duplication (TGAAC)
within the inverted repeat portion of the IS; IS1294 bp 344
missing in homology |
S0064 |
402 |
94% id over 341 aa |
IS1294 bp 1353-1659 missing and replaced by
332 bp of non-homologous sequence |
S0065 |
76 |
55% id over 35 aa to IS1650 transposase |
homology to IS1294 region in pUB2380, bp 5397-5499. Part of S0065 is located outside the IS1294
sequence; S0065 aa 35-70 of 76 are 56% identical to aa 111-146 of
148 of IS1650 transposase [Streptomyces coelicolor A3(2),
see GB: AL117669] |
S0075 |
55 |
portion of IS1294 |
S0075-S0077 = complete IS element; target site duplication
(TGAAC) within the inverted repeat portion of the IS; IS1294
bp 344 missing in homology |
S0076 |
63 |
portion of IS1294 |
|
S0077 |
399 |
95% id over 345 aa |
|
S0095 |
402 |
94% id over 341 aa |
IS1294 bp 1353-1659 missing and replaced by
332 bp of non-homologous sequence |
S0173 |
400 |
95% id over 345 aa |
complete IS element; target site duplication (GAAC) |
S0281 |
398 |
94% id over 345 aa |
IS1294 bp 1-274 missing |
Total IS1294 ORFs: 9, complete
elements: 3 |
|
IS1328
Y. enterocolitica, 1359 bp [GB: Z48244]
transposase: 334 aa [GB: CAA88289] |
S0215 |
217 |
71% id over 217 aa, from aa 106-322 |
no DNA homology seen except to IS1328 bp 991-1049;
new transposase? |
Total IS1328 ORFs: 1 |
|
IS1353
IS1353 is found in integron In2, which is found in Tn21;
Tn21 was originally described in Shigella flexneri
1613 bp [GB: U40482]
ORFA: 202 aa [GB: AAC44291]
ORFB: 304 aa [GB: AAC44292]
translational fusion protein: 514 aa [GB:
BAA78795] |
S0125 |
80 |
56% id over 80 aa of fusion protein |
no DNA homology to IS1353 |
S0126 |
94 |
73% id over 53 aa of the fusion protein |
|
Total IS1353 ORFs: 2 |
|
Total known IS ORfs: 114, complete elements:
20 |
Name and description of IS element |
pWR501 ORF ID |
size (aa) |
homology to orf in known IS element |
additional comments regarding homology &target
sequence |
ISSfl1
929 bp; 20 bp IR, 3 bp DR
ORF homologies to Streptomyces coelicolor IS1650 ORFs,
but no nucleotide homology to IS1650 [GB: AL117669, bp 6054-6951]; IS1650 has two ORFs, possibly encoding
a transposase via translational frameshifting:
IS1650 orfA: 136 aa [GB: CAB56132]
IS1650 orfB: 148 aa [GB: CAB56133] |
S0078 |
110 |
48% id to orfA over 77 aa |
S0078-S0079 constitutes bp 16-869 of ISSfl1 |
S0079 |
172 |
44% id to orfB over 139 aa |
|
S0101 |
171 |
43% id to orfB over 139 aa |
S0101-S0102 constitutes bp 4-827 of ISSfl1 |
S0102 |
180 |
45% id to orfA over 101 aa |
|
S0203 |
171 |
44% id to orfB over 139 aa |
S0203-S204 constitutes complete ISSfl1 with
duplication of TA at target site |
S0204 |
149 |
46% id to orfA over 115 aa |
|
Total ISSfl1 ORFs: 6, complete
elements: 1 |
|
ISSfl2
1373-1376 bp; 8 bp IR: CCCCCCAC
ORF homology to Streptomyces coelicolor IS110 ORF,
but no nucleotide homology to IS110 [GB: Y00434]
IS110 major ORF (site specific recombinase?):
405 aa [GB: CAB51528]
|
S0055 |
398 |
58% id over 391 aa, aa 3-393 |
|
S0128 |
399 |
59% id over 391 aa, from aa 3-393 |
|
Total ISSfl2 ORFs: 2, complete
elements: 2 |
|
ISSfl3
1301 bp; 18 bp IR: CTGAGGGATCCCCACAAA, 9 bp DR: CCCTTCAGG
ORF homology to IS10 transposase: 402 aa [GB: I67760]; DR homologous to bp 756-764 of Y. pestis IS100
|
S0034 |
397 |
33% identity over 361 aa |
|
Total ISSf13 ORFs: 1, complete
elements: 1 |
|
ISSfl4
2728 bp; 11 bp IR, no DR seen
ISSfl4 is a member of the IS66 family; it is composed
of 3 ORFs with homologies to the ISEc8 ORFs of EHEC 0157:H7
strain EDL933:
ORF L0013: 133 aa [GB: AAC31492]
ORF L0014: 115 aa [GB: AAC31493]
ORF L0015: 512 aa [GB: AAC31494] |
S0008 |
183 |
51% id over 178 aa from aa 32-210 of L0015 |
|
S0023 |
112 |
36% id over 104 aa from aa 32-132 of L0013 |
|
S0024 |
34 |
73% id over 33 aa from aa 1-33 of L0015 |
|
S0025 |
435 |
56% id to 380 aa from aa 26-406 of L0015 |
|
S0072 |
198 |
65% id over 133 aa from aa 377-509 of L0015 |
|
S0081 |
83 |
59% id to 32 aa from aa 435-466 of L0015 |
|
S0090 |
119 |
62% id over 164 aa from aa 3-166 of L0015 |
|
S0116 |
350 |
65% id over 340 aa from aa 166-508 of L0015 |
has IR sequence GTAAGCGCCCC upstream of start codon |
S0117 |
190 |
38% id to 185 aa from aa 1-163 of L0015 |
S0116-S0119 constitutes a complete ISSfl4 sequence
|
S0118 |
114 |
69% id over 83 aa from aa 1-81 of L0014 |
|
S0119 |
224 |
40% id over 52 aa from aa 8-59 of L0013 |
has IR sequence GGGGCGCTTAC downstream of stop codon |
S0216 |
350 |
65% over 340 aa from aa 166-508 of L0015 |
has IR sequence GTAAGCGCCCC downstream of stop codon |
S0217 |
190 |
39% id over 185 aa from aa 1-163 of L0015 |
S0216-S0219 constitutes a complete ISSfl4 sequence |
S0218 |
117 |
68% id over 83 aa from aa 1-81 of L0014 |
|
S0219 |
224 |
40% id over 52 aa from aa 8-59 of L0013 |
has IR sequence GGGGCGCTTAC upstream of start codon |
Total ISSfl4 ORFs: 16, complete
elements: 2 |
|
Total New IS ORFs: 25, complete elements:
6 |
|
Known + New IS ORFs: 140, complete elements:
26 |
bacterial spp. & database ID# |
pWR501 ORF ID |
size (aa) |
homology to database sequence |
comments regarding homology to database sequences |
E. coli transposase: 406 aa [GB:
AAF60967] |
S0014 |
124 |
87% id over 74 aa from aa 333-406 |
|
S0015 |
125 |
86% id over 111 aa from aa 220-330 |
also shows 32% id over 93 aa to aa 227-312 from A.
eutrophus ISAE1 transposase [406 aa; GB: AAC13658] |
|
ETEC 92 kb plasmid, IS1414 orf:
402 aa [GB: AAG18473] |
S0027 |
52 |
63% id over 36 aa from aa 1-31 |
S0027-S0028 is frameshifted and show homology to the
same set of sequences in the database |
S0028 |
62 |
70% id over 62 aa from aa 36-67 |
|
|
uropathogenic E. coli, orfB: 195 aa [GB:
AAC61729] |
S0073 |
130 |
99% id over 130 aa from aa 66-195 |
S0073 also shows 34% identity over 126 aa to E.
coli IS21 IstB ATP binding protein [265 aa; GB:
P15026],
40% id over 115 aa from aa 123-237 to IS5376 from Bacillus
stearothermophilus [251 aa; GB: CAA48046],
and to Rhizobium proteins Y4sD/Y4nD/Y4iQ |
|
E. coli IS10 transposase: 402 aa [GB:
I67760] |
S0110 |
111 |
36% id over 74 aa |
|
|
E. coli genome, Kohara clone o230#3: 96 aa [GB:
BAA35811]
|
S0247 |
91 |
94% id over 18 aa from aa 2-18 |
beyond 1-17 aa, S0247 is 42% id over 47 aa to putative
transposases from Rhizobium (Y4bL, Y4tB, Y4kJ: 516 aa; GB:
P55379);
S0247 also shows homology to S0021, S0242 (91% id over 59 aa) and
to S0119 and S0219 (51% id over 35 aa) |
|
E. coli IS21 tnp: 390 aa [GB:
CAA32898] |
S0127 |
316 |
84% id over 57 aa |
|
|
Mesorhizobium loti transposase: 347 aa [GB:
NP_105082] |
S0178 |
112 |
46% id to 103 aa from aa 241-343 |
S0178 also shows 31% id over 110 aa to ISSfl2
orf S0055 (and S0128), and 38% id over 107 aa to Pseudomonas atlantica
IS492 (318 aa) |
|
A. eutrophus ISAE1 transposase [406 aa; GB:
AAC13658] |
S0183 |
76 |
46% id over 46 aa from aa 212-255 |
start codon GTG of S0183 is embedded within the 3'
end of an IS91 element; S0183 also shows homology to tnp/Thiobacillus,
urf/p0157, putative tnp/Nocardia |
|
Rhizobium sp. NG234 putative transposase
Y4bL (Y4kJ, Y4tb): 516 aa [GB: AAB91627] |
S0021 |
94 |
46% id over 72 aa from aa 15-86 |
S0021 and S0242 also show homology to aa 17-75 of S0247 |
S0242 |
80 |
46% id to 52 aa from aa 35-86 |
|
|
Mesorhizobium loti probable transposase: 185
aa [GB: NP_102655] |
S0041 |
121 |
44% id over 111 aa, from 2-112 aa |
also shows 41% id over 113 aa to IS1111A/IS1328/IS1533
family transposase from Caulobacter crescentus (354 aa; GB:
AAK24705),
36% id over 113 aa to Rhizobium sp. NGR234 putative transposase
Y4pF/Y4sB (387 aa; GB: AAB91816/AAB91842)
|
|
E. coli 0157:H7 EDL933 ISEc8-related
ORF: 537 aa; [GB: AAG55306] |
S0038 |
104 |
80% id over 45 aa from aa 111-155 |
also shows 52% id over 42 aa to L0015, aa 91-132 [L0015:
512 aa; AAC31494] |
E. coli 0157:H7 EDL933 ISEc8-related
ORF: 285 aa; [GB: AAG55307] |
S0230 |
189 |
98% id over 80 aa from aa 198-277 |
also shows 34% id over 182 aa to L0015, aa 196-374
[L0015: 512 aa; AAC31494] |
E. coli 0157:H7 EDL933 ISEc8-related
ORF: 161 aa; [GB: AAG55303] |
S0231 |
126 |
95% id over 126 aa from aa 36-161 |
also shows 40% id over 102 aa to L0015, aa 387-504
[L0015: 512 aa; AAC31494] |
|
Total Unknown IS ORFs: 15 |
|
Known + New + Unknown IS ORFs: 153 |