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Bacterial Pathogen Genome Sequencing Projects The genome center at the University of Wisconsin was established to sequence the genome of Escherichia coli K-12 strain MG1655, which has served for decades as a model organism for basic studies of biochemistry, physiology, genetics and biotechnology. When the sequencing of this genome was completed in 1997, we turned to a group of related bacterial pathogens, making use of the E. coli K-12 sequence to accelerate analysis of the new genomes. The pathogenic Enterobacteriaceae we have selected include members of E. coli human diarrheagenic and extraintestinal strains, Yersinia pestis, Shigella flexneri, and Salmonella Typhi. Analysis of the enterohaemorrhagic E. coli O157:H7 EDL933 genome was the first of these to be completed, and serves as a model for our comparative studies. The results of genome sequencing support the widespread involvement of horizontal transfer in the evolution of the Enterobacteriaceae, leading to the presence of distinct islands of DNA in different lineages. Since virulence determinants, as well as "backbone" genes, are shared among pathogens, we expect this multigenomic approach to lead to characterizing a gene pool of virulence determinants - the "pathosphere." Availability of sequenced strains Most of the strains we are sequencing have been deposited with the American Type Culture Collection (ATCC); genomic DNA is also available from these strains:
Current Status of Sequencing Projects
*Plasmids from several Enterobacteriaceae are also being sequenced as separate projects. All sequence data for the genome sequencing projects was generated by the Genetics Sequencing and Genomics Services Center (GSGSC)
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2002-2024 UW E. coli Genome Project
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