GROWTH OF E.coli STRAIN MG1655

Growth of MG1655 in M9 minimal medium

MG1655 has a doubling time of 67 minutes on M9 minimal medium + glucose + thiamine (1ug/ml). This is calculated from the following data from 4 separate growth curves. MG1655 recently obtained from ATCC (#47076) is included for comparison: (click to enlarge images)

 

time (hours)

OD600 in 0.4% Glu 3/1/01

time (hours) OD600 in 0.4% Glu 2/23/01 time (hours) OD600 in 0.2% Glu 1/24/01 time (hours) OD600 using ATCC 47076 0.2% Glu 10/20/00 OD600 in 0.2% Glu 10/20/00
0 0.015 0 0.014 0 0.005 0 0.006 0.005
1 0.025 1 0.019 1 0.005 2 0.011 0.008
2 0.039 2 0.033 2 0.012 3 0.013 0.011
3 0.071 3 0.062 3.25 0.026 5 0.057 0.052
4 0.131 4 0.125 4.5 0.054 6 0.112 0.106
5 0.227 5 0.231 5.5 0.103 7 0.203 0.193
6 0.368 6 0.436 7 0.211 8 0.327 0.31
7 0.634 7.5 0.99 8 0.327
9 0.493
log2 at 2 -4.680382066 log2 at 2 -4.921390165 log2 at 2 -6.380821784 log2 at 5 -4.13289427 -4.26
log2 at 6 -1.442222329 log2 at 6 -1.19759996 log2 at 5.5 -3.279283757 log2 at 7 -2.300448367 -2.37
doublings / hour 0.81 doublings / hour 0.93 doublings / hour 0.89 doublings / hour 0.92 0.95
minutes doubling time 74.12 minutes doubling time 64.45 minutes doubling time 67.71 minutes doubling time 65.49 63.42

 


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