LOCUS EZTN5KM 1221 bp DNA linear SYN 01-NOV-2020 DEFINITION Synthetic transposon EZ-Tn5 , complete sequence ACCESSION . VERSION . KEYWORDS . SOURCE synthetic transposon EZ-Tn5 ORGANISM synthetic transposon EZ-Tn5 other sequences; artificial sequences; vectors. REFERENCE 1 (Mosaic Ends) AUTHORS Zhou,M., Bhasin,A. and Reznikoff,W.S. TITLE Molecular genetic analysis of transposaseŠend DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase JOURNAL J. Mol. Biol. 276, 913Š925 (1998) PUBMED 9566196 REFERENCE 2 (Description of EZ-Tn5) AUTHORS Goryshin,I.Y., Jendrisak,J., Hoffman,L.M., Meis,R. and Reznikoff,W.S. TITLE Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes JOURNAL Nat. Biotechnol. 18(1), 97-100 (2000) PUBMED 10625401 COMMENT EZ-Tn5 is a trademark of Lucigen Corporation. FEATURES Location/Qualifiers source 1..1221 /organism="synthetic transposon EZ-Tn5 " /mol_type="other DNA" repeat_region order(1..19,1203..1221) /note="ME (hyperactive Mosaic End)" /rpt_type=inverted primer_bind complement(43..57) /note="KAN-2 RP-1 Reverse Primer" gene 138..953 /gene="nptI" CDS 138..953 /note="kanamycin resistance gene" /gene="nptI" /gene_synonym="aph(3')-Ia" /gene_synonym="neo" /gene_synonym="aphA1" /codon_start=1 /transl_table=11 /product="Aminoglycoside 3'-phosphotransferase" /EC_number="2.7.1.95" /note="APH(3')-I; Kanamycin kinase, type I; Neomycin-kanamycin phosphotransferase type I; APH(3')I" /function="confers resistance to neomycin, kanamycin, paromomycin, neamine, paromamine, vistamycin, gentamicin A, and G418 (Geneticin(R))" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1127..1151 /note="KAN-2 FP-1 Forward Primer" ORIGIN 1 CTGTCTCTTA TACACATCTC AACCATCATC GATGAATTGT GTCTCAAAAT CTCTGATGTT 61 ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA 121 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCTTGCTCG AGGCCGCGAT 181 TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC 241 AATCAGGTGC GACAATCTAT CGATTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA 301 AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC 361 TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT 421 GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG 481 ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC 541 CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC 601 GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG 661 TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA 721 CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA 781 TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT 841 GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA 901 ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT 961 TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGGCTTTG 1021 TTGAATAAAT CGAACTTTTG CTGAGTTGAA GGATCAGATC ACGCATCTTC CCGACAACGC 1081 AGACCGTTCC GTGGCAAAGC AAAAGTTCAA AATCACCAAC TGGTCCACCT ACAACAAAGC 1141 TCTCATCAAC CGTGGCGGGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTTCAGGGT 1201 TGAGATGTGT ATAAGAGACA G //