Our group at the University of Wisconsin - Madison sequenced the Escherichia coli K-12 genome, and continue to maintain and update its annotation. We created knockouts of many genes, archive clones of many ORFs, and an extensive gene expression data set under a variety of physiological conditions. These data sets are made available freely to the community.

   We also sequenced six pathogenic enterobacterial strains, related to E. coli K-12, as well as a number of plasmids. The comparative genomics aim of this effort is to characterize the gene pool of horizontally transferred elements and virulence determinants known as the pathosphere.

   The ASAP database, developed in collaboration with Dr. Nicole Perna, is a platform for community sharing of annotation information and data which is made available for downloading by the community.