About the Functional Genomics Project
We are using the whole genome sequence of E. coli K-12 strain MG1655 to address how the over 4000 genes of this organism act together to enable its survival in a wide range of environments. At least something can now be said about the function of ~80% of the genes, but their full biological role and the regulatory pathways that control their expression is only beginning to be probed.
E. coli Movies As part of
a study of E. coli carbon source utilization*, we have examined
cell motility using video microscopy of log phase cultures. Movies (.mpeg
files) of cultures grown in MOPS minimal medium supplemented with six
different carbon sources are available for downloading:
* this study has now been published: M. Liu, T. Durfee, J. E. Cabrera, K. Zhao, D. J. Jin, and F. R. Blattner (2005) Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J Biol Chem 280(16): 15921-15927 [PubMed]
To aid in constructing a comprehensive biological framework for E. coli, we are developing genome-wide resources and data sets for use by the community. Resources include:
A set of archive clones with each ORF cloned in a standard vector.
Systematic mutagenesis: A strain collection consisting of a mutant in every gene.
DNA microarray experiments of wild type and mutants under different conditions.
Phenotypic analysis of each mutant strain.
Protocols and recipes used in each project above.
For more information about the K-12 MG1655 strain, see strain information.
© 2002-2014 UW E. coli Genome Project