We sequenced the E. coli K-12 genome, and continue to maintain and update its annotation. We created knockouts of many genes, archive clones of many ORFs, and an extensive gene expression data set under a variety of physiological conditions. These data sets are made available freely to the community.
We also sequenced six additional enterobacterial strains which are pathogens related to E. coli K-12, and many plasmids. The comparative genomics aim of this effort is to characterize the gene pool of horizontally transferred elements and virulence determinants known as the pathosphere.
The ASAP database, developed in collaboration with Nicole Perna, is a platform for community sharing of annotation information and data which is made available for downloading by the community.
October 12-13, 2012 72nd Annual Meeting, North Central Branch of ASM Fargo, ND
September 16-20, 2012 19th Annual International Meeting on Microbial Genomics Lake Arrowhead, CA
May 30, 2012 5th Annual Oliver Smithies Symposium Madison, WI
